There are 12 items available. Estimated between Tue. Similar re- sults were obtained with primary hip- pocampal neurons, which endogenously express mZIP4 Fig. The protein kinases that were analyzed, ERK, JNK and p38, become phosphorylated and activated under excitotoxic conditions. In our previous work, we had inhibited general endocytosis as well as the low-density lipoprotein receptor-related protein LRP, which acts as a scavenger receptor for tPAand de- termined that these mechanisms were not responsible for zinc import in the pres- ence of tPA. You can change your ad preferences anytime. TheinactivemutantSAtPAwasusedasanegativecontrol data not shown and its densitometric value since it is catalytically inactive was subtracted from the values of all the other recombinant proteins, following which the binding for each mutant was normalized to that observed for the wild-type protein. Since the physiological requirement for zinc transport could vary dramatically according to localized neuronal activity, and since relatively little is known about how the ZIPs are regulated, we hypothesized that cells might control their capacity for zinc import by using transcriptional mechanisms.
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disease-causing mutations on ZIP4, a representative LIV-1 protein.
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Here we based sequence analysis also suggests a common dimeric Mono-Q column and then by size exclusion chromatography (GE Healthcare). The.
Correspondence should be addressed to Stella E.
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Mol Neurobiol — In non- stimulated extracellular space e. Any international shipping and import charges are paid in part to Pitney Bowes Inc. Hi, if we buy now, when you shipped to me?
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Intracellular pH changes or the presence. Download Table | Common non-synonymous SNPs in the ZIP4 (SLC39A4) gene. from Electronic supplementary material The online version of this article.
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Shipping to: Worldwide. Learn More - opens in a new window or tab International shipping and import charges paid to Pitney Bowes Inc. To further explore the localization of intracellularly imported zinc, we used FluoZin-3 loading and two-photon mi- croscopy. Nat Biotechnol — To explore this possibility in the context of zinc toxicity, we probed several sig- naling pathways known to be involved in pro- and antiapoptotic outcomes for neurons.
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To examine the intracellular zinc lev- els, cellular fluorescence was read at excita- tion nm and emission nm on a fluorescent plate reader. J Comp Neurol —
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Packaging should be the same as what is found in a retail store, unless the item was packaged by the manufacturer in non-retail packaging, such as an unprinted box or plastic bag. Learn more. Brain Res — MichaelFrohmanforcriticaleditingofthemanuscript, aswellasDr.