Vortex, then microcentrifuge for 30 sec. Brunet, A. B The phosphorylation and protein expression of AKT was investigated by immunoblotting. Collect cells by centrifugation and aspirate supernatant. Figure 1.
Polyclonal Antibody for studying Akt1 (Ser) phosphate/Akt2 (Ser) phosphate/Akt3 (Ser) phosphate in the PI3K / Akt Signaling research area. Western blot analysis of extracts from PC-3 cells, untreated or LY/wortmannin-treated, and NIH/3T3 cells, serum-starved or PDGF-treated, using Phospho-Akt (Ser) (D9E) XP® Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb # (lower).
CST PhosphoAkt (Ser) (D9E) XP® Rabbit mAb
Works great for rat skeletal muscle. Polyclonal Antibody for studying Akt1/Akt2/Akt3 in the PI3K / Akt Signaling research Phospho-GSK-3β (Ser9) Antibody # was used to confirm downstream.
Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.
Analyze cells in DNA staining solution on flow cytometer. Preparing Cell Lysates Aspirate media. Incubate for at least 5 min at room temperature.
AKT total + Phospho S FLOW Kit (ab) Abcam
Proceed with detection Section D.
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Diese Seite auf Deutsch anzeigen. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Cantley, L.
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Brunet, A. J Biol Chem. For best results, allow mountant to cure overnight at room temperature.
I am using an 8% acrylamide gel and the antibody was diluted in 5%. Overview. Product name. AKT total + Phospho S FLOW Kit.
CST PhosphoAkt (Ser) Antibody
Detection method. Fluorescent. Sample type.
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Adherent cells, Suspension cells. Assay type. Direct. Phospho-AKT (Ser) antibody (Cell Signaling Technologies, #). Remove the media and re-suspend the cells in 10 ml of fresh media.
Discard supernatant in appropriate waste container. Bone Marrow Transplant 48 3 —8. Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis Aliquot desired number of cells into tubes or wells.
Nat Rev Immunol 3 4 —
home > Cell Signaling Technology > Phospho-Akt (Thr) Antibody Suspension survival mediated by PP2A-STAT3-Col XVII determines tumour initiation and.
Analysis of p-Akt protein levels in cells in suspension yielded. antibodies ( S) were purchased from Cell Signaling Technology, Inc. (CST.
Diehl, J. The supernatant is the cell lysate. Wash sections three times with wash buffer for 5 min each.
To Purchase S View sizes. Cell Signaling Technology product webpage.
Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film. Pre-clear enough lysate for test samples and isotype controls.