The failure of bone remodeling can lead to pathological conditions of bone structure and function. Abstract Background The structure and function of bone tissue is maintained through a constant remodeling process, which is maintained by the balance between osteoblasts and osteoclasts. Cytotoxicity measurement and cell proliferation assay The effects of remifentanil on both cell viability and proliferation were examined by the well-established colorimetric 3- 4,5-dimethylthiazol -2,5-diphenyltetrazolium bromide MTT, Sigma-Aldrich assay. Androgen and estrogen dynamics in women with vertebral crush fractures. Services on Demand Journal. Thereafter, cell-cell fusion of pre-OCs occurs and multinucleated osteoclasts mature osteoclasts are formed [ 25 ]. J Bone Miner Res. Cell-cell fusion.
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Disclosures: C.A.M. Kuluk. None. T In the cyclic group, PTH returned to baseline during off-cycles and declined again during on-cycles.
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Bone resorption by osteoclasts. Int Immunopharmacol. Marked decline in serum concentrations of adrenal C19 sex steroids precursors and conjugated androgen metabolites during aging.
Each experiment was performed in duplicate and three separate experiments were performed for each treatment group. J Dent Anesth Pain Med. Therefore, it can be administered via continuous infusion during general anesthesia and sedation in the intensive care unit ICU [ 1213 ].
In this study, we evaluated the effects of remifentanil on pre-OC differentiation and the expression of osteoclastogenic genes and transcription factors.
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|The MTT assay indicated that the cell viability of pre-OCs was not affected by treatment with various concentrations of remifentanil 0.
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Clin Biochem. In a previous in vitro study, remifentanil suppressed the RANKL-induced osteoclast differentiation data not shown.
As previous results showed that remifentanil upregulated pre-OC differentiation Fig. Hormone predictors of bone mineral density changes during the menopausal transition. Osteoclast cell fusion: mechanisms and molecules. Protective effects of remifentanil against H2O2-induced oxidative stress in human osteoblasts.
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However, the effect of remifentanil on osteoclasts has not been studied. Find articles by Eun-Jung Kim. Am J Obstet Gynecol. In brief, pre-OCs were seeded in well plates with the indicated concentrations of remifentanil 0. The association of plasma androgen levels with breast, ovarian, and endometrial cancer risk factors among postmenopausal women.
Effect of remifentanil on preosteoclast cell differentiation in vitro
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|Therefore, osteoclastogenic cell fusion is an essential component of the process of osteoclastogenesis.
Pre-OCs were obtained by culturing bone marrow-derived macrophages BMMs in osteoclastogenic medium for 2 days and then treated with various concentration of remifentanil. Open in a separate window. Effects of estrogen replacement on the progression of coronary artery atherosclerosis. These findings suggested pre-OC migration enhanced cell fusion, which resulted in an increase in osteoclast size. The climateric ovary as as a functional gonadotropin-driven androgen-producing gland.
Our goal was to detect the endometrial volume change in conception cycles. Around days 21–22 of the conception cycle, a blastocyst implants and . Lorenzo Botto; J. David Erickson.
The hormone hCG also supplements LH during the menstrual cycle [16,17] and promotes organ development in the fetus during gestation [18, 19]. with SYBR green PCR master mix (Applied Biosystems) for 40 cycles of 15 s at 95℃ . Hauge EM, Qvesel D, Eriksen EF, Mosekilde L, Melsen F. Cancellous bone.
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Kular J, Tickner J, Chim SM, Xu J. An overview of the regulation of bone .
The cells became adherent after 3—4 days of culture and were used as BMMs, the osteoclast precursor cells.
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Statistical analysis Data were obtained from at least three independent experiments. Therefore, osteoclastogenic cell fusion is an essential component of the process of osteoclastogenesis.
In brief, pre-OCs were seeded in well plates with the indicated concentrations of remifentanil 0. Find articles by Ji-Uk Yoon. Estrogen deficiency and risk of Alzheimer's disease in women.
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Find articles by Eun-Jung Kim. During orthognathic surgery or prolonged sedation in ICU, the continuous infusion of remifentanil may affect the bone physiology of the patient, because the patients who undergo orthognathic surgery or are treated in the ICU are more likely to have pathologic bone disease or to be elderly. The reversal phase is a transition from osteoclast to osteoblast activity. Comparison of the effects of estrogen alone and estrogen plus androgen on biochemical markers of bone formation and resorption in postmenopausal women.